melting point GCTCGTAGCTACGT - Wolfram|Alpha
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Assuming the input refers to a formula | Use "melting point" as a formula instead
monovalent salt ion concentration:  
magnesium ion concentration:  
primer concentration:  

Input information:

link to /input/?i=0.05+moles+per+liter&lk=1link to /input/?i=0.05+moles+per+liter&lk=1link to /input/?i=0.05+moles+per+liter&lk=1link to /input/?i=0.05+moles+per+liter&lk=1link to /input/?i=2.5+millimoles+per+liter&lk=1link to /input/?i=2.5+millimoles+per+liter&lk=1link to /input/?i=2.5+millimoles+per+liter&lk=1link to /input/?i=2.5+millimoles+per+liter&lk=1link to /input/?i=0.4+micromoles+per+liter&lk=1link to /input/?i=0.4+micromoles+per+liter&lk=1link to /input/?i=0.4+micromoles+per+liter&lk=1link to /input/?i=0.4+micromoles+per+liter&lk=1
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Result:

link to /input/?i=58.3+degrees+Celsius&lk=1link to /input/?i=58.3+degrees+Celsius&lk=1
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Equation:

Salt concentration dependence:

Framelink to /input/?i=1+millimolar&lk=1link to /input/?i=1+millimolar&lk=1link to /input/?i=2+millimolar&lk=1link to /input/?i=2+millimolar&lk=1link to /input/?i=3+millimolar&lk=1link to /input/?i=3+millimolar&lk=1link to /input/?i=4+millimolar&lk=1link to /input/?i=4+millimolar&lk=1link to /input/?i=1+millimolar&lk=1link to /input/?i=1+millimolar&lk=1link to /input/?i=4+millimolar&lk=1link to /input/?i=4+millimolar&lk=1
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Length:

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Amino acid sequence:

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Download full output as:

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